Composite

Part:BBa_K1166002:Design

Designed by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero   Group: iGEM13_TecMonterrey   (2013-09-17)


HlyA-tag+Secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1306


Design Notes

HlyA C terminal part has a 5' section compatible with Silver Fusion standard. Downstream, HlyB and HlyD are both under the AraC/pBAD promoter to be induced by arabinose.


Source

The HlyB and HlyD sequences come from the parts registry, while the HlyA from the registry had mayor problems, so we took it from the genomic sequence.

References